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Results Effects of cuprizone feed formulation on body weight Following 3 weeks on diets of control powder, cuprizone-containing powder, control pellet or cuprizone-containing pellet, mice were assessed for weight loss, a widely recognised hallmark of cuprizone intoxication 34

The increased glucose (25 mM) was used to inhibit -cell glucagon secretion to diminish glucagon levels to basal levels to improve the dynamic range of the assay and thus effectively unmask the role of ligand-mediated glucagon secretion as described earlier 45