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Description
Isolation and partial characterization of Borrelia burgdorferi inner and outer membranes by using isopycnic centrifugation
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The prepared slides were baked, followed by dewaxing, rehydration, staining (employing HE, Safranin O, and immunofluorescence for HIF-1, IL-6, IL-1, TNF-, NF-B(P65)), dehydration, clearing, and mounting
The corrected genomic fragments were validated with quantitative PCR with primers described in Supplemental Table 7
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