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Even the overall expression of cyclins (Cyclin D, Cyclin E, CDK 4, CDK 1, and PCNA) was down-regulated in time-dependent increasing concertation of the EGCG-treated group as compared to the untreated control group by Western blot analysis that blocked the G1 phase of the cell cycle [47]

doi: 10.3389/fphar.2022.1036140 75 LuoLXuG

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Cells were transferred to 0.5 mL sonication tubes and lysed using a Q800R3 Sonicator (QSonica, 3 cycles at 50% amplitude, 20 seconds ON, 20 seconds OFF, at 4 C)