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Funding This work was supported by National Natural Science Foundation of China (81771140), Youth Medical Talent Program of Jiangsu Province (QNRC2016068), and Medical Innovation Team of Jiangsu Province (CXTDA2017030)
In these methods, enzyme variants are encoded by DNA templates that can be generated by a variety of means, including from commercial gene synthesis, error-prone PCR, and saturation mutagenesis
Gronningsaeter IS, Reikvam H, Aasebo E, Bartaula-Brevik S, Tvedt TH, Bruserud O, et al
doi: 10.1016/j.chom.2017.01.005 310 KimM-SRohSWBaeJ-W
It's simple, Erick explains
