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Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort

Using the MTT assay (a standard test for cell viability), they measured whether AHK-Cu made these cells multiply faster

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Although serum levels of ALT in the vitamin B12 group decreased significantly, this change did not reach a significant level compared to the placebo group

Lyophilization helps preserve peptide stability during storage and allows researchers to reconstitute the material for controlled laboratory experimentation