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The reducing agent has a maximum absorbance at 340 nm, and the GP4-specific enzyme activity can be calculated by measuring non-specific enzyme activity and total enzyme activity by adding GPX-4 inhibitor to the system
This again suggests the critical role of peroxynitriterather than LPOas the primary cause of oxidative damage to mitochondrial DNA 18 and protein nitration, which are central mechanisms of toxicity (Fig
Another approach comes from Eli Lilly themselves
10.1038/nrm3311 63 HensJ
