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The samples were centrifuged (13,400 rpm) for 30 min, and the supernatant was collected and filtered (0.2 m syringe filter) into an LCMS vial

Carcinogenesis 31:18331843 Sayin VI, LeBoeuf SE, Singh SX, Davidson SM, Biancur D, Guzelhan BS, Alvarez SW, Wu WL, Karakousi TR, Zavitsanou AM, Ubriaco J, Muir A, Karagiannis D, Morris PJ, Thomas CJ, Possemato R, Vander Heiden MG, Papagiannakopoulos T (2017) Activation of the NRF2 antioxidant program generates an imbalance in central carbon metabolism in cancer

However, some PDAC cells exhibit reduced sensitivity to erastin-induced ferroptosis

As with anything related to government agencies, this doesnt happen overnight

Preferred sites: Abdomen (avoid 2 inches around navel) Front or outer thigh Upper arm (posterior) Technique: Clean injection site with alcohol swab Let alcohol dry completely Pinch fold of skin Insert needle at 45-90 degree angle Inject slowly over 5-10 seconds Release skin, withdraw needle Light pressure if any bleeding Rotate sites to prevent tissue irritation