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These scaffolds relied on diffusional release of the protein from the fibrin gels, rather than covalently linking the protein to the gel as discussed in the Section Fibrin. This makes them an unattractive approach for delivery of pro-angiogenic peptides, as the release of the drug is governed by diffusion (Figure 2A) rather than degradation, and would likely result in accelerated release of pro-angiogenic peptides

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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

Make the necessary changes and press F9 to allow the adjustment to continue processing

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