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Cells were plated on top of the 0.6% Bacto agar layer in glucose and glutamine free DMEM media containing 10 mM glucose and 4 mM glutamine and supplemented with 10% FS and 0.3% Bacto Agar

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The ability of GP to decrease TMAO levels suggests that GP has an inhibitory effect on the pathway of phosphatidylcholine to TMAO
