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CYP27A1 effectively catalyzes the first rate-limiting step in the alternative pathway, while 7-hydroxysteroid 7-hydroxylase (CYP7B1) participates in subsequent 7-hydroxylation, responsible for the majority of CDCA synthesis (Rao et al., 2008)

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They act at extrasynaptic receptors, facilitating tonic inhibition (Chen et al., 2019