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Cells were washed three times for 5 min in PBS/azide, then incubated for 2 h at room temperature with shaking in 1% NDS, 0.1% Triton X-100 in PBS/azide with the corresponding secondary antibodies (donkey anti-goat conjugated to Alexa Fluor 488, Invitrogen, A-110055, 1:500
The assay is based on the conversion of citrate to isocitrate, to -ketoglutarate, which results in the production of NADPH
doi: 10.1002/cac2.12250 86 SpornMBLibyKT
While multiple studies highlight the anti-photoaging effects of N
Protein was finally imaged with the Licor IRDye 800CW streptavidin on the Odyssey Sa imager
