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Mineva EM, Sternberg MR, Zhang M, Aoki Y, Storandt R, Bailey RL, Pfeiffer CM

As antifols are schedule dependent, preliminary experiments are aimed at defining the longest duration of exposure that would allow for continuous logarithmic phase growth of cells without changing of the culture media while maintaining a linear relationship between SRB optical density and cell number

The cell lines used in the present study were purchased from American Type Culture Collection (Manassas, VA, USA), DS Pharma Biomedical (Osaka, Japan), and Horizon Discovery Ltd