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Estrogen-related receptor (ERR) and ERR are essential coordinators of cardiac metabolism and function

The final and crucial activation step occurs predominantly within tumor tissues, where thymidine phosphorylaseoften overexpressed in malignant cellsconverts 5-DFUR into the active compound 5-fluorouracil (5-FU)

The Kinase-Glo Reagent is then added to terminate the PKA reaction and detect the remaining ATP via a luciferase reaction

Beads were washed four times with NT2 buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM MgCl 2 , 0.05% NP-40) and RNA extracted and quantified as previously described 76,115,116

Arch Biochem Biophys 397:384391 Rochette L, Ghibu S, Richard C, Zeller M, Cottin Y, Vergely C (2013) Direct and indirect antioxidant properties of -lipoic acid and therapeutic potential