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Avoid repeated temperature cycling
Litvan et al., 2012

Yousefifard, M., Hosseini, M., Oraii, A., Vaccaro, A.R., Rahimi-Movaghar, V.: Assessment of severity of impairment in spinal cord injury
Elife 5, 2016

The maximum absolute values for the mean and median were situated at the 1 h time point for upregulated genes but at the 9 h and 30 min time points respectively for the down regulated genes (Table 2)
