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White blood cells (WBCs) were counted using a Neubauer hemocytometer, with blood diluted using Natt-Herrick as a diluent following (44)

doi: 10.1111/jcpt.12280

[DOI] [PMC free article] [PubMed] [Google Scholar] 68.Kleemann R, Verschuren L, Morrison M, Zadelaar S, van Erk MJ, Wielinga PY, Kooistra T

Alcohol is a particular concern, as it's thought to substantially lower the body's levels of this antioxidant

Sections of 5 m were stained with Hematoxylin and Eosin (H&E) for general tissue morphology, where hematoxylin imparted a blue hue to the nuclei while eosin stained the cytoplasm pink
