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When NNMT activity is inhibited, these methyl donors become available for other methylation reactions in the body

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Reactions were performed with 5 M histone H3 (histone L-arginine) substrate, 1 M radiolabeled S-adenosyl-L-[methyl- 3 H] methionine (SAM) co-substrate, and recombinant human PRMT3 enzyme

Pregnant women aged 18 y who presented for prenatal care before or at 14 wk of gestational age (as judged by the date of the last menstrual period) were eligible for inclusion