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PMID: 23715506
Recent trial demonstrated that widefield OCTA detected peripheral non-perfusion in patients with early NPDR, improving risk stratification, as well as identify central and peripheral neurovascular and microstructural impairments in patients with full-course DR (124, 125)
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Then, the RNA quality was evaluated by checking A260/A280 with Nanodrop TM OneC spectrophotometer (Thermo Fisher Scientific Inc.)
The confluent coverslips were then aseptically transferred to new six well plates containing 900 serum free DMEM
