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Salt is helpful in preventing contaminating proteins from binding nonspecifically to the nickel agarose beads and to your his-tagged protein of interest

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tigurinus ) making up 25.91%, 12.37%, and 8.06% of the microbial community, respectively

PLoS One 7 , e44278 (2012)
Moreover, in vitro studies indicated APOE4-treated apoE / neurons exhibited more phosphorylated tau proteins than APOE3- and APOE2-treated neurons