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High-performance liquid chromatography analysis remains the gold standard for detecting impurities below 1%

a-c , Difference in abundance in the extracellular cysteinylglycine in medium (a) and relative abundance of intracellular cysteinylglycine (b) and intracellular cysteine (c) for HCC-1806 cells grown in the indicated conditions and time points (n = 3 technical replicates representative from 2 independent experiments, except for c at 24 h, in which n = 2 technical replicates from 2 independent experiments)

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Then, the equivalent cDNAs were synthesized using the PrimeScript RT reagent kit (Takara Bio) according to the manufacturers standard protocol