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Although aging is the strongest predictor of ovarian redox decline, environmental and lifestyle factors can further accelerate oxidative stress

Before the measurement started, the volunteers gave their written informed consent according to the local research ethics regulations, the current version of the Declaration of Helsinki, DIN EN ISO 14 155 and were approved by the Institutional Review Board of the University of Tbingen

The absorbance was read at 570 nM using a LUMIstar Omega luminometer (BMG Labtech)

We used Deming regression implemented in the deming v.1.4 package in R to assess the relationship between effect sizes for these lead SNPs in cross-ancestry meta-analyses in the two biobanks while accounting for standard errors in both 83,84

As an osmoprotectant, betaine primarily protects cells from osmotic/ionic stress by regulating the concentration and volume of intracellular fluid