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The animals were randomly distributed to receive saline (NaCl 0.9%0.1 mL/300 g of body weight) or HgCl 2 through intramuscular injection (first dose of 4.6 g/kg, subsequent doses of 0.07 g/kg/day) for 4 weeks, as shown in Figure 1

Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) for TGF-1 mRNA Total RNA was extracted from frozen lung tissues with TRIzol reagent (TaKaRa Biotechnology Co., Ltd., Dalian, China) and was reverse-transcribed using a reverse transcription kit (TaKaRa Biotechnology Co., Ltd., Dalian, China)

Drug Discovery Today (2017) 22(5):796804

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On the other hand, laser treatments tend to be pricier, with a broader range of Rs 4,000 to Rs 30,000, depending on the type and extent of treatment required