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An Afe I restriction site directly upstream of the Strep -tag II, together with the unique Xba I restriction site in front of the whole reading frame, was subsequently used to exchange the entire coding region for sfGFP by the ones for different other POIs (see Supplementary Table S1)

Integrated multiomic analysis reveals the high-fat diet induced activation of the MAPK signaling and inflammation associated metabolic cascades via histone modification in adipose tissues

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The RSD of six replicate injections was calculated and assay precision was represented as the %RSD

Biswas SK, Rahman I