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Analysis of MRAB Biofilm Architecture Using Confocal Laser Scanning Microscopy (CLSM) To initiate biofilm growth, 500 L MRAB-3 (OD 600 = 0.5 0.05) in TSB was added to microscope glass slides and incubated at 37C in a static incubator for 48 h

Moreover, this indicated that both, the extension of the reaction time and the increase of the excess of reducing agent, do not affect the decrease of N-Hcy-Lys signal

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After centrifugation with 18,000 g at 4C for 30 min, the supernatant was loaded on SPE columns (Phenomenex strata-X, 300 mg) that were preconditioned with 1 ml of methanol and 1 ml of water

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