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Description
Ni-INDIGO beads (20 l) (Cube Biotech, 75105) were added and excess peptide was removed using a PD MidiTRAP G-25 (Cytiva) followed by size-exclusion chromatography (Superdex 75) in 25 mM HEPES, 150 mM NaCl and 1 mM TCEP, pH 7.5

Sepesi B, Schmidt HM, Lada M, Walsh GL, Mehran RJ, Rice DC , Roth JA, Vaporciyan AA, Ajani JA, Watson TJ, Swisher SG, Low DE, Hofstetter WL

Cancer Cell 2016;30:96885.ArticlePubMedPMC 90
Cytoprotection was originally defined as a property to defend the cells against different noxious agents, an effect pointed out in gastric mucosa as a gastric acid independent effect

It is the most abundant protein in mammals
