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How AOD-9604 Works (in Brief) AOD-9604 was engineered from the C-terminal segment of human growth hormone (amino acids 177191) the portion thought to influence fat metabolism. In preclinical models, this fragment supported fat oxidation and lipid mobilization without the broader hormonal effects of full-length hGH. Because it does not activate the traditional hGH receptor, its fat-metabolism effects are considered indirect and receptor-independent. Discovery & Research Milestones Originally developed in the 1990s as a potential anti-obesity drug

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Antibody production and quality control His-GspD and His-PilQ Olut were expressed in Escherichia coli BL21 cells and purified using Nickel-NTA agarose beads (Qiagen, Germantown, MD) according to the manufacturers instructions

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