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Paired-end Fastq files (R1: cell barcode reads, R2: RNA reads) were processed with Cutadapt (v4.1) for adapter trimming, quality filtering, and phase-shift base removal

Improving CAR-T cell persistence through metabolic engineering, including the modulation of SIRT1 and PRMT5, has also demonstrated promise in sustaining antitumor activity

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Moreover, p16, p53, and p21 have important functions in the DNA-damage repair pathways which are among the most frequently compromised pathways in pathological conditions such as tumor growth ( 1 stage of the cell cycle ( e.g