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To reduce the urea concentration, the samples were diluted 5-fold with ammonium bicarbonate (AMBIC) before overnight digestion at 37 C with trypsin (type IX-S from Porcine Pancreas) using a 1:30 (w/w) enzyme to substrate ratio
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12 For example, dopamine denervation in the SN significantly reduces NSC proliferation in the SVZ and SGZ in mice, whereas pharmacologic D2 receptor stimulation significantly recovers NSC proliferation
7B and Supplementary Figure S4)
