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Description
Membranes were incubated overnight at 4 C with Cleaved Caspase-3 (Asp175) (Cell Signalling Technology) and with peroxidase-linked anti-rabbit IgG or anti-mouse IgG secondary antibodies (GE Healthcare Life science) for 1 h at room temperature
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As reported by the company, enrolled patients experienced no severe adverse events, and the patients showed increasing improvements in fine and gross motor skills, communication, autonomic function, and seizure activity
The presence of GI problems in individuals with ASD can complicate behavioral management and may exacerbate core symptoms of autism, such as communication difficulties and repetitive behaviors[21]
Nat Rev Urol 18(7):387403
