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To explore the underlying mechanisms of elevated histone lactylation in GSCs, we measured levels of key metabolites in GSCs and DGCs, including products of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle, by liquid chromatographymass spectrometry (LC-MS), which revealed upregulation of glycolytic metabolites, including lactate, in GSCs (Figure 2, A and B)

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