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The following day, cells were cultured in 5 ml of fresh YPDA medium with a starting optical density at 600 nm (OD 600 ) of 0.3 and incubated until the OD 600 reached 0.81.0

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After use, itching symptoms can be relieved within hours to days, and the rash area and severity score are significantly reduced

Toncev G, Milicic B, Toncev S, Samardzic G