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The supernatant was collected, mixed with 20 L ice-cold trichloroacetic acid, incubated on ice for 10 min and centrifuged at 12,000 g for 5 min

M.JanelidzeS.BrundinL.et al

C5ar 1 / mice colony was maintained in the animal care facility of the Ribeirao Preto Medical School of Ribeirao Preto, University of Sao Paulo

( f ) Representative images (processed with Imaris for display purposes only) of spleen sections from nave mice or mice infected with MCMV or vCD44BP (day 2 PI) stained with anti-B220, anti-CD8 and anti-Xcr1 mAbs

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