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The participants were allocated to the two groups using blocked randomization and a computer-generated list of random numbers that had been prepared by an investigator with no involvement in this study

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Since there was no significant difference of the mRNA level of GCS-HS and GS between control and proline group at any time points, it can be assumed that the activity of GCS in proline group might be upregulated by the upregulation of regulatory subunit of GCS, leading to the significant upregluation of total GSH from 6 h to 12 h

ROS may also mediate metabolism reprogramming ( Classical strategies include direct/indirect HIF targeting and interference in upstream/downstream HIF regulators/signaling pathways

This distinction is crucial not only for ethical practice but for legal protection in academic and commercial research environments