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Chang CJ, Mulholland DJ, Valamehr B, Mosessian S, Sellers WR, Wu H (2008) PTEN nuclear localization is regulated by oxidative stress and mediates p53-dependent tumor suppression

[Abstract]2022 Apr;26(7):2089-2103

It will introduce bacteria and a host of other contaminants that will ruin your experiment

When concentrations are in the range between four times and one fourth of the C 50 , the time course of effect is essentially a linear function of time
