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The PCR primer sequences used in qRT-PCR are provided in Supplementary Table S1
CellTiter-Glo reagent (3 mL) was diluted in 9 mL of DPBS, and 100 L of this solution was added to each well on top of culture media

This knowledge guides appropriate mitigation strategies

While immune cells in the TIME regulate cancer cell proliferation, cancer cells, in turn, can influence TIME by secreting signaling molecules that induce immune tolerance
