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The membranes were incubated with a primary antibody solution (1:1000 in TBST with 5% BSA) overnight and with HRP-conjugated secondary antibodies (1:5000) for 1 h

The study by Vaes et al (56) emphasizes the heterogeneous nature of CFS, and that different subtypes may reflect differences in the mechanisms of fatigue development, which may guide the formulation of individualized treatment plans, accuracy of prognostic assessment and clinical stratified management (Tables III and IV)

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