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Its k cat / K m values toward tyrosine and DOPA are 600 and 181 s 1 mM 1 , respectively, which are 2.78- and 1.79-fold higher than the WT enzyme
After three rinses in PBS (10 min each), the tissues were incubated with secondary antibodies in PBS containing 5% BSA at room temperature for 2 h, washed three times with PBS, mounted using Mowiol mounting medium, and imaged under a fluorescence microscope
& Miano, J
