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Lung tissue was stored in RNAlater and homogenized in TRIzol using a TissueLyser II (Qiagen, Toronto, Ontario, Canada)

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The mixture was pre-incubated for 5 min in a shaking water bath at 37 C

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Figure 5 Discussion A variety of eryptosis inducers have been used to study eryptosis of nave erythrocytes over the past 20 years

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Scholars have mostly evaluated the inhibitory and apoptotic effects of different concentrations of active ingredients on different cells by MTT assay and SRB method

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One common feature to both animal models of SOD1 ALS and human disease is the deposition of aggregate SOD1 protein in neuronal and glial inclusions (Philips & Rothstein, 2015;Stieber et al., 2000

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