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In response to increased plasma Hcy concentrations, there may be vascular endothelial dysfunction, reduced expression of thrombomodulin and activated protein C [64], a decrease in the presence of anticoagulant heparans [65], and a decrease in tissue plasminogen activator receptors on the surface of vascular endothelial cells [66]

Lactate dehydrogenase (LDH) assay Cytotoxicity Detection Kit (LDH Kit Plus, Roche, Basel, Switzerland) was used to determine cell death in EA.hy 926 cells, as described by the manufacturer

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Cameron AD, Sinning I, LHermite G, Olin B, Board PG, Mannervik B, et al

PRE-084 as a tool to uncover potential therapeutic applications for selective sigma-1 receptor activation