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The molecule isnt known to produce tolerance or downregulation that would require breaks

Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions

Differential reactivity of germ line allelic variants of a broadly neutralizing HIV-1 antibody to a gp41 fusion intermediate conformation

HEK293T cells were seeded at 700,000 cells per well in a 6-wells plate for 16 h
