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We identified 386 metabolites that significantly differentiated between HPV_C and HPV_PH groups, of which 364 were associated with VM components such as Lactobacillus, Hoylesella, Fannyhessea and Megasphaera

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To simplify purification, affinity purification tags are often fused to a recombinant protein of interest.The most popular tag is the polyhistidine (6xHis) tag.Some proteins, like eukaryotic proteins may not exhibit proper protein activity or folding if expressed in E.coli

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It is involved in the transport of fatty acids into the mitochondria, where they are metabolized to produce energy

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