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This alteration suppressed GATA3 expression via the bile acid-Takeda G protein-coupled receptor 5(TGR5)-cyclic adenosine monophosphate (cAMP) signaling pathway, leading to a marked ~90% reduction in IL-22 secretion from intestinal group 3 innate lymphoid cells (ILC3s), which subsequently induced insulin resistance, ovarian dysfunction (characterized by increased follicular cysts and decreased corpora lutea), and impaired fertility (82)

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Each indication requires its own evidence base

Finally, although this study focused on a single induction protocol, variations in differentiation methods across research groups may affect maturation timelines, limiting the direct applicability of findings to alternate protocols

Ellwanger et al., 2008)