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Therefore, to an amber screw-cap HPLC vial was added in the following order: methanol, triethylamine (800 equiv., c fin = 80 mM), keto-enal stock solution (50 equiv., c fin = 5 mM) and amino-modified oligonucleotide (1 equiv., c fin = 200 M)

Lpez Snchez, Elena Dragioti, Selin Woo, Dong Keon Yon Heliyon.2024

[DOI] [PMC free article] [PubMed] [Google Scholar] 261.Colilla M., Vallet-Reg M

doi: 10.3389/fmolb.2021.7 47 Coz YatacoA
Deconvolution of these two peaks revealed both native and monoglutathionylated M pro as expected and contained an estimated 35% monoglutathionylated M pro and less than 5% diglutathionylated M pro , with the remaining M pro unmodified (Figure 4B)
