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doi: 10.1016/j.sbi.2019.03.001

Repeated freeze-thaw cycles should be avoided to preserve the integrity of the copper-peptide complex and ensure consistent experimental results

27 Other studies used patients with all types of thyroid cancers in their study

This suggests that excessive GHK-Cu (or other copper peptides) might theoretically push skin MMP-1 concentrations beyond levels supporting healthy collagen production into ranges associated with collagen fragmentation and breakdown seen in older skin with naturally elevated MMP-1