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After injection, bound proteins were eluted with a linear gradient from 0 to 100% elution buffer (20 mM Tris, 2 M NaCl, pH 7.0) over 10 column volumes (CV) at 1 mL/min

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The finding of premature senescence of balding DPCs in association with the expression of markers of oxidative stress and DNA damage suggests that balding DPCs are particularly sensitive to environmental stress