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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing

In: Proceedings of the national academy of sciences, vol 100

doi: 10.2147/dddt.s3225

resources, Y
This layer has been given several names over the years, but lately it is commonly referred to as the paraneural sheath of the sciatic nerve
