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In the meantime, four protons are translocated across the membrane aiding the proton gradient

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The intestinal tract is a major site of aGvHD, representing the majority of morbidity/mortality, and is key in amplifying systemic aGvHD [40]

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Cleanup is achieved using ultrafiltration [31], acid precipitation with trichloroacetic acid [28, 37, 41, 50] or perchloric acid [29, 34, 45], and organic solvent precipitation with methanol [48, 51, 52] or acetonitrile [31, 53]