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Description
Besides undergoing 7-dehydroxylation during colonic transit, the hydroxy groups on the steroid nucleus may be modified by dehydrogenation, epimerization, or even elimination to form an unsaturated bile acid (with a double bond in the steroid nucleus)
Gao, X.-J

monocytogenes EGDe Genome and annotation

The macromolecular receptor was processed using AutoDockTools 1.5.7 software, including the addition of hydrogen bonds, calculation of charges, setting of rigid structures, and designation as the receptor, and then exported in PDBQT format

This testing matters because manufacturing processes can introduce harmful substances
