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Gdf11 depletion decreases the number of newborn neurons in the adult hippocampus To test the hypothesis that Gdf11 depletion leads to fewer newborn neurons, we performed a pulse chase experiment using the thymidine analog bromo-deoxyuridine (BrdU)

These include two-photon microscopy [53], confocal laser scanning microscopy (CLSM) [21], and Raman spectroscopy [54,55]

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This method bypasses the digestive system, ensuring that the body absorbs the maximum amount of nutrients