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On the other hand, for TP53, the interacting residues Cys220, Glu221, Ser227, Asp228, and Cys229 are within the DNA-binding domain (residues 102292), specifically the L3 loop (residues ~ 220230), which is vital for structural stability and DNA interaction 111,112

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In contrast, cyclic hydroxylamines like CMH are cell-permeable and can be preincubated directly within intact tissue