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Description
Prepare the HisLink Binding, Wash and Elution Buffers Note: Polyhistidine-tagged proteins can be eluted using 2501,000mM imidazole
A thio-sugar auxiliary, incorporated as a glycosyl amino acid during solid-phase peptide synthesis, facilitates amide bond formation near the ligation site via thiol exchange with a peptidyl thioester
Adapted from Wadden et al
At 24 h after plating the cells were stained as described in Materials and Methods and observed by confocal microscopy in the chamber provided with 5% CO 2 and at 37C
The detection wavelength was 220 nm and the injection volume was 5 L
